At present, most enterprises use plate and frame, centrifugation and other methods to remove bacteria and some macromolecular impurities in the fermentation broth. The feed liquid separated in this way has high content of soluble impurities, large feed liquid volume, and low feed liquid clarity, resulting in low efficiency of purification methods such as resin or extraction in the subsequent process, which in turn increases production costs. “Bona Bio” successfully applied membrane separation technology to the production process of impurity removal and purification of fermentation broth, successfully solved the problems of separation, purification and concentration in the industrialized production of fermentation broth, and at the same time achieved the purpose of energy saving, consumption reduction and clean production. It provides economical, advanced and reasonable solutions for fermentation enterprises.
Bona membrane separation technology advantages:
1. The high precision of membrane filtration ensures the clarification effect of the biological fermentation liquid, which has great advantages compared with the traditional process, the impurity removal is thorough, and the product quality is obviously improved.
2. Membrane filtration is carried out in a closed environment, with a high degree of automation, and the filtration process reduces the waste of fermentation broth and pollution to products.
3. The membrane filtration process can operates at normal temperature (25°C), no phase change, qualitative change, no chemical reaction, no damage to active ingredients, no damage to heat-sensitive ingredients, and greatly reduces energy consumption.
4. Membrane filtration process, the mycelium can be recovered while clarifying, removing impurities, concentrating and purifying the product;
5. The membrane concentration equipment has large flux, fast concentration speed, and stable and reliable process;
6. Membrane concentration has high filtration precision, and the filtered liquid has high purity. It can be considered for reuse in production, which reduces sewage discharge and has good environmental protection significance;
7. The degree of automation is high, safe and reliable, effectively reducing labor intensity, and the membrane filtration process is carried out in a closed container to achieve clean production;
8. The membrane element has a large filling area and a small area of the system, which is convenient for technological transformation, expansion or new projects of old factories, which can effectively reduce production costs and investment.
Now, the editor of Shandong Bona Group will introduce the application of membrane separation technology in biological fermentation broth.
1. Application in post-treatment of antibiotics
There are by-products, residual medium and soluble protein in the penicillin fermentation filtrate, which will cause emulsification during extraction. It is difficult to separate the aqueous phase and the ester phase, which affects the transfer of penicillin between the two phases, prolongs the extraction process time, and reduces the concentration of penicillin in the extraction section and yield. Treatment of penicillin fermentation broth with ultrafiltration membrane can effectively remove protein and other macromolecular impurities and eliminate emulsification during extraction. After ultrafiltration, all soluble proteins are retained, and the total yield of ultrafiltration and extraction of penicillin is basically the same as the original extraction yield, and phase separation is easy to achieve during extraction, which reduces solvent loss, does not need to add demulsifier, and reduces costs.
2. Application in post-processing of vitamins
Vitamin C is a typical vitamin product produced by fermentation. A lot of research has been done on the treatment of Vc fermentation broth with membrane technology, and industrialization has been successfully realized already. Vc is fermented by sorbitol under the action of bacteria to form the intermediate gulonic acid, which is further transformed and produced after purification. Gulonic acid fermentation broth is pretreated to remove solid impurities and some proteins, and then ultrafiltration to remove macromolecular impurities such as proteins and polysaccharides, purifying the feed liquid entering the next step of ion exchange, increasing the exchange rate of the ion exchange column and reducing the regeneration liquid and Washing water consumption, thereby reducing the one-step ion exchange process and saving energy. If treated with reverse osmosis membrane, most of the water in the raw material liquid can be removed, instead of the first-level concentration and evaporation process in production. The adoption of membrane technology shortens the process of proto-gulonic acid extraction, reduces the amount of acid-base regeneration waste liquid and cleaning water, and reduces the thermal decomposition loss of gulonic acid during the concentration process, reducing production costs.
3. Application in amino acid post-processing
Monosodium glutamate wastewater belongs to high-concentration refractory organic wastewater, which not only has high organic content, but also contains high NH4+ and SO4^2-. It is difficult for traditional biological treatment technology to make it meet the standard discharge. Ultrafiltration membrane is used to remove bacteria and bacteria in monosodium glutamate wastewater. Macromolecular protein and other components, the removal rate of SS in wastewater can reach more than 99%, and the removal rate of CODcr is about 30%, which can reduce the processing load of biological method and recover protein in wastewater..
Membrane separation technology has the advantages of simple equipment, convenient operation, high processing efficiency and energy saving, and has been widely used in various fields. With the advancement of science and technology, membrane separation technology will be continuously improved and applied to more industries.
Post time: Apr-20-2022