Application of Ultrafiltration in Protein Purification

Protein concentration ultrafiltration technology1

With our industry advantages and a lot of practical experience, Shandong Bona Group adopts advanced ultrafiltration membrane technology and membrane concentration technology, which can purify and concentrate proteins effectively. Since the membrane concentration is a low temperature concentration, the energy consumption of the concentration less than traditional process, and the damage to the heat-sensitive components of the product would be less also. In addition, membrane concentration uses the principle of mechanical sieving to intercept enzymes, allowing small molecular impurities and water to pass through. Therefore, during the concentration process, inorganic salts and small molecular impurities in the enzymatic hydrolysis solution can be removed effectively, reducing bitterness and residual agricultural chemicals . Today, the editor of Shandong Bona Group will introduce the application of ultrafiltration in protein concentration.

Disadvantages of proteins extracted by traditional enzymatic method:
1. The volume of the extract is large and the production cycle of the product is long.
2. Incomplete impurity removal of enzymatic hydrolysate affects the quality of collagen.
3. The finished products often have bitter and fishy taste and poor taste.
4. The filtration level is rough, and the water solubility of the product is poor.

The method of ultrafiltration technology to produce isolated protein can fundamentally change the traditional alkali-acid precipitation and water washing method, and can greatly improve the product quality. In this way, the isolated protein can be separated, purified and concentrated without phase transition, effectively avoiding the increase in salt content due to repeated denaturation in the acid-base adjustment process in the traditional process, greatly improving the protein purity (up to 92%) and reducing the ash content (≤4.0 %).

Advantages of protein concentration ultrafiltration technology:
1. The membrane system has the characteristics of high separation efficiency. It is used for the clarification, sterilization, impurity removal and filtration of the raw liquid. It can completely remove the macromolecular tannin, pectin, mechanical particle impurities, foreign matters and various microorganisms that affect the product quality in the raw liquid, and the resulting product has good and stable quality.
2. It not only realizes the sterilization, impurity removal and filtration of raw material solution, but also realizes the separation of macromolecular substances and small molecular substances at room temperature.
3. Compared with the traditional process, the membrane system can realize long-term and stable continuous industrial production, and the restorability performance of the system is good.
4. Concentrated at room temperature, the biological activity of protein basically no affect.

Shandong Bona Group is a professional manufacturer in the production of membrane separation equipment. we have many years of production and technical experience, focusing on solving the problem of filtration and concentration in the production process of biological fermentation/alcoholic beverages/Chinese medicine extraction/animal and plant extraction. Circular production methods can help customers improve production efficiency and achieve cleaner production effectively . If you faced problems in membrane filtration, please feel free to contact us, we will arrange the professional technicians to serve you.


Post time: Apr-20-2022
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